Exploiting the inherent promiscuity of the acyl transferase of the stambomycin polyketide synthase for the mutasynthesis of analogues

Chem. Sci., 2025, 16,5076-5088
DOI: 10.1039/D4SC06976E, Edge Article

Open Access

&nbsp This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Li Su, Yaouba Souaibou, Laurence Hôtel, Christophe Jacob, Peter Grün, Yan-Ni Shi, Alicia Chateau, Sophie Pinel, Helge B. Bode, Bertrand Aigle, Kira J. Weissman
The acyl transferase (AT) of module 12 of the stambomycin polyketide synthase (PKS) exhibits unusual substrate tolerance. This promiscuity was exploited to generate multiple new bioactive stambomycin macrolides by extender unit-based mutasynthesis.
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